Estabilidad de la glucosa oxidasa en sistemas amorfos formados por los disacáridos sacarosa, maltosa y trehalosa

Abstract

GLUCOSE OXIDASE STABILITY IN AMORPHOUS SYSTEMS FORMED BY SACCHAROSE, MALTOSE AND TREHALOSE DISACCHARIDES. Glucose-oxidase (GOD), suffers conformational change during freeze-drying. In order to determine the protection level granted by amorphous matrices (AM) of saccharose, maltose, trehalose and their combinations, the thermal inactivation constants (KD ) of GOD trapped in these systems were determined. For its evaluation, GOD samples were balanced at different water activities and heated up to 30, 50 and 70 ºC. The best AM found for GOD stability was saccharose-trehalose (5/10% p/v). The KD values (KD.10-4 ) at aw = 0.0 were 3 at 30 ºC and 6 at 70 ºC. For non-protected GOD under the same conditions these values were 48 at 30 ºC and 257 at 70 ºC