Immunoglobulin subclass in experimental murine Toxocara cati infection

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Abstract

Aim: The aim of this study was to detect specific immunoglobulin (Ig) that could be used to determine monoclonal antibody in conjugate-making an effort for the indirect enzyme-linked immunosorbent assay (ELISA) diagnostic kit of toxocariasis in human. Materials and Methods: The study was conducted to assess the Ig profile, based on ELISA-isotyping, in mice infected with second stage larvae eggs of Toxocara cati. The optical density values of anti-T. cati mice serum IgG subclasses were analyzed by applying ANOVA factorial. Results: The specific IgG subclass in mice infected with T. cati mice was found to be IgG2β. Conclusion: Subclass of IgG, especially IgG2β, can provide leads about the use of the monoclonal antibody in conjugate making an effort for the indirect ELISA diagnostic kit.

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Kusnoto, Koesdarto, S., Mumpuni, S., & Prasetyo, R. H. (2017). Immunoglobulin subclass in experimental murine Toxocara cati infection. Veterinary World, 10(11), 1333–1336. https://doi.org/10.14202/vetworld.2017.1333-1336

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