Methods to study the role of ectomycorrhizal fungi in forest carbon cycling 2: Ergosterol analysis method to quantify the fungal content in ectomycorrhizal fine roots

Abstract

At the first report of this series, we reconfirmed the importance of the study to quantify the role of mycorrhizal fungi in forest carbon cycling. In this report, we focused on the ergosterol analysis method, a general method to quantify the biomass of fungi in ectomycorrhizal fine roots, and its procedure was detail described. Concentration of the ergosterol in ectomycorrhizal fungi is used as a conversion factor and this value has the great influence on the estimation of fungal biomass in ectomycorrhizal fine roots. We reviewed the data about conversion factor and recognized that the necessity of further data accumulations. Keywords: biochemical indicator, ergosterol, fine root, fungal biomass, phospholipid fatty acid (PLFA) •X-Ñ ‚Ì 'Y 'f •z ŠÂ‚É ‚¨‚¯‚¨‚¯ ‚é ŠO• ¶ ‹Û •ª ‹Û ‚Ì-ð Š" ‚ð OE¤‹ † ‚· ‚é Žè-@2:ŠO • ¶ ‹Û•ª ‚Ì ‹Û-Þ ŠÜ-Ê ‚ð 'è-Ê ‰»‚· ‚é ƒGƒ‹ ƒS ƒXƒe ƒ• •[ ƒ‹ •ª •Í Žè-@:-¢'º '½•• "ü(•L " ‡ 'å Šw'å Šw‰@ • ¶ •¨ OE-‰ÈŠwOE¤‹ † ‰È),‹´-‹´-{-õ('Ñ •L '{ ŽY'å ŠwŠÂ‹« '• • ‡ ‰È Šw•u •À),-Ø ‰º •W•F(•L " ‡ 'å Šw'å Šw‰@ • ¶ •¨ OE-‰È ŠwOE¤‹ † ‰È),-x ‰z •F-Y(•L " ‡ 'å Šw'å Šw‰@ '• • ‡ ‰È ŠwOE¤‹ † ‰È)-v Ž|: ‚± ‚Ì ƒVƒŠ•[ ƒY‚Ì 'aêê 'e ‚Å‚Í,•X-Ñ ‚Ì 'Y 'f •z ŠÂ‚É ‚¨‚¢‚¨‚¢ ‚Ä‹Û•ª ‹Û ‚Ì 'è-Ê ‚ª •d-v ‚Å‚ ‚é ‚± ‚AE‚ð •ÄŠm"F ‚µ ‚½¡‚± ‚Ì 'ae "ñ'e ‚AE‚È ‚é •ñ •• ‚Å‚Í,ŠO • ¶ ‹Û•ª ‚Ì OE`•¬ ‚³ ‚ê ‚½•A •¨ •× •ª ‚Ì ' † ‚Ì ‹Û-Þ ƒo ƒCƒI ƒ} ƒX‚Ì 'è-Ê ‚Ì •Û‚É-p ‚¢ ‚ç‚ê ‚éˆê‚éˆê "Ê "I ‚È Žè-@‚Å ‚ ‚é ƒGƒ‹ ƒSƒXƒe ƒ• •[ ƒ‹ •ª •Í-@‚É •Å"_ ‚ð ‚ ‚Ä,‚» ‚Ì Žè• ‡ ‚ð •Ú•q ‚· ‚é¡ŠO • ¶ ‹Û•ª ‹Û‚Ì ƒGƒ‹ ƒSƒXƒe ƒ• •[ ƒ‹ "Z"x ‚Í •Ï Š· OEW •" ‚AE‚µ ‚Ä-p‚¢ ‚ç‚ê,‚» ‚Ì 'l ‚ª •ª ‚Ì ' † ‚Ì ŠO• ¶ ‹Û•ª ‹Û‚Ì ƒo ƒCƒI ƒ} ƒX‚ð •" 'è 'l ‚É-^‚¦ ‚é ‰e ‹¿ ‚Í 'å ‚« ‚¢¡‰ä •X‚Í •Ï Š· OEW •" ‚Ì 'l ‚ð ƒOEƒr ƒ… •[ ‚µ,•X ‚È ‚é ƒf •[ ƒ^‚Ì '~ •Ï ‚ª •K-v‚Å‚ ‚é ‚± ‚AE‚ð "F Ž¯ ‚µ ‚½¡ ƒL•[ ƒ•-ƒh:ƒG ƒ‹ ƒSƒXƒe ƒ• •[ ƒ‹,‹Û-Þ ‚Ì ƒo ƒCƒI ƒ} ƒX,•× •ª,• ¶ ‰» ŠwŽw•W,ƒŠ ƒ"Ž‰ Ž¿Ž‰-b Ž_(PLFA)