Synthesisandcharacterization of nanocompounds Fe3 O4/Ag: Its effect against Enterobacter aerogenes and Enterococcus faecalis

Abstract

In the present study, we report the synthesis and characterization of magnetite/silver magnetic nanoparticles (Fe3 O4 /Ag) and the study of their antibacterial properties against the bacteria Enterobacter aerogenes (Gram-negative) and Enterococcus faecalis (Gram-positive). Magnetite magnetic nanoparticles (MNPs) were prepared by a sol-gel method using cetyltrimethylammonium bromide (CTAB), as a surfactant, followed by in-situ chemical reduction of silver ions by glucose, and polyvinylpyrrolidone (PVP), as a dispersive agent, to obtain the antibacterial nanocomposite of Fe3 O4 /Ag. Morphological and spectroscopic studies of magnetite nanoparticles and nanocomposite were carried out using the Fourier transform infrared spectroscopy (FTIR), Raman spectroscopy, Mössbauer spectroscopy (MS), X-ray diffraction (XRD), scanning electron microscopy (SEM), and energy dispersive X-ray spectroscopy (EDS). Spherical Fe3 O4 MNPs showed an average diameter of 10 nm and the average size for nanocomposite was 28 nm. The antibacterial test indicated that the use of the nanocomposite of Fe3 O4 /Ag at a concentration of 5 mg·mL-1 allowed a total inhibition of the growth of the microorganisms studied from an initial concentration of 108 bacteria mL-1.