Free radicals scavenging activity and oxidative DNA damage protecting property of methanol extract from honeycrisp apple

Abstract

Objectives: This research reports the qualitative phytochemical constituents and considers the in vitro free radicals scavenging activities based on DPPH and nitric oxide assays and oxidative DNA damage protection activity of methanol extract from Honeycrisp apple. The foremost interest for this research was to use standard measures to determine nitric oxide scavenging activity, DPPH-scavenging activity and DNA damage protecting activity to assess the antioxidant potential of methanol extract from the apple. Materials and Methods: Concentrations of apple extracts with the intervals 20 µg/ml to 100 µmg/ml were prepared and mixed with suitable volumes of reagents and the corresponding absorbances read at the respective wavelength. Results: The outcomes of this research specify that methanol extract of Honeycrisp apple contain multiple phytochemical compounds that can expertly shield the body against oxidative stress caused by free radicals and might then be used as a source of potent natural antioxidant compounds. The IC50 values of methanol extract from Honeycrisp apple varied from 10.30 to 36.29 µg/ml which indicates the high radical scavenging activity of the sample. Conclusion: The DNA damage defensive potential of the extracts was also revealed, which could be used in cancer inhibition.