An updated liquid chromatographic assay for the determination of glyphosate in technical material and formulations

Abstract

An updated liquid chromatographic (LC) method for the determination of glyphosate in technical material and formulations was tested by 7 laboratories. Samples were dissolved in water and injected into an LC system with a C18 guard column, an anion exchange analytical column, and a phosphate buffer mobile phase with 12 to 15% methanol. Detection was by UV absorption at 195 nm or refractive index. Automated injections were made. Calculations were based on peak area comparisons with external standards. The interlaboratory study analyzed 6 matched pairs of 3 glyphosate technical materials and 3 glyphosate formulations. The matched pair results were analyzed by using the AOAC spreadsheet and Youden's matched pair calculations. Coefficients of variation for the 6 samples ranged from 0.46 to 0.85%.