Effects of oxygen concentration on the expression of an antioxidant gene in HepG2

Abstract

To clarify the effects of O2 concentration cO 2) on antioxidant gene expression in human hepatocytes, mRNA expression of HepG2 cells cultured at 1, 3, and 5% cO2 and atmospheric gas-phase, was measured. The expression of some genes fluctuated depending on the cO2 in the incubator. This indicates that cO2 is a critically important factor in the investigation of human biological mechanisms.