Antimicrobial Effect of Silver Nanoparticles Synthesised with Ocimum Sanctum Leaf Extract on Periodontal Pathogens

Abstract

Nano silver particles have been widely used in biomedical applications for diagnosis and treatment. Green synthesis of nanoparicles by using Ocimum sanctum results in rapid, economical and ecofriendly product for drug delivery. The minimum inhibitory concentration and minimum bactericidal concentration tests detected the antimicrobial action of pure Ocimum sanctum extract (OSE), silver nitrate (SN), and silver nanoparticles synthesized with OSE (AgNP) against four periodontal pathogens i.e., Fusobacterium nucleatum (Fn), Porphyromonas gingivalis (Pg), Aggregatibacter actinomycetemcomitens (Aa) and Prevotella intermedia (Pi). The results suggest that, all the test solutions reveal their sensitivity to test microorganism in a dose dependent manner. Hence, increase in the concentration may improve their antimicrobial action. On comparision AgNP found to have an enhanced antimicrobial activity and Aa found to be more susceptible for the same.