Five tetramic acid derivatives isolated from the iranian fungus colpoma quercinum cctu a372

Abstract

Submerged mycelial cultures of the ascomycete Colpoma quercinum CCTU A372 were found to produce five previously undescribed tetramic acids, for which we propose the trivial names colposetins A–C (1–3) and colpomenoic acids A and B (4 and 5), along with the known compounds penicillide (6) and monodictyphenone (7). The planar structures of 1–5 were determined by high-resolution electrospray ionization mass spectrometry (HR-ESIMS) and extensive 1D and 2D nuclear magnetic resonance (NMR) spectroscopy. Their absolute configurations were determined by the combination of electronic circular dischroism (ECD) analysis, J-based configurational analy-sis, and a rotating-frame Overhauser effect spectroscopy (ROESY) experiment. Colposetin B dis-played weak antimicrobial activity against Bacillus subtilis and Mucor hiemalis (MIC 67 µg/mL).