In Vitro Study of Anti-Elastase and Antioxidant Activities on Mabolo (Diospyros blancoi) Leaf and Stem Bark Extracts

Abstract

The mabolo plant (Diospyros blancoi A. DC.) has anti-aging potential. This research aimed to investigate the anti-elastase and antioxidant properties of mabolo leaf extract (LE) and stem bark extract (SBE). Using the ultrasound-assisted extraction method, the method used started with the extraction of mabolo plant parts of leaves and stem bark with methanol solvent. Yields and phytochemical screens were determined for each extract and tested for Total Phenolics Content (TPC), Total Flavonoid Content (TFC), anti-elastase and antioxidant [2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,20-azinobis (3-ethylbenzothiazo-line-6-sulfonic acid) diammonium (ABTS) free radical scavenging methods and ferric reducing power (FRAP) method] activities. According to the findings, LE and SBE have strong anti-elastase and antioxidant activities with 50% inhibitory concentration (IC50) values below 50 μg/mL. LE and SBE had anti-elastase IC50 values of 41.06 and 26.52 μg/mL, respectively. The IC50 value for DPPH and ABTS in LE were 4.34 and 2.95 μg/mL, whereas in SBE they were 3.73 and 1.96 μg/mL. LE and SBE had FRAP values of 6308.61 and 6835.29 μmol/g, respectively. In conclusion, the findings of this investigation revealed that LE and SBE exhibited strong anti-elastase and antioxidant properties. Furthermore, SBE demonstrated stronger anti-elastase and antioxidant activity than LE. As a result of these findings, the mabolo plant has the potential to be developed as an anti-aging agent.