Production of Bioethanol from Pulverized Japanese Cedar Powder by Simultaneous Saccharification and Fermentation with High-temperature Fermentability Yeast Schizosaccharomyces japonicus SS4-5

Abstract

Akita Research Institute of Food and Brewing 4-26, Aza-Sanuki, Araya-machi, Akita-shi, Akita 010-1623, Japan The present study was concerned with bioethanol production from the pulverized Japanese cedar powder by simultaneous saccharification and fermentation (SSF). The pulverized Japanese cedar powder, which was treated with vibration grained named tandem-ring mill, could be efficiently converted into sugar in enzymatic saccarification. When SF was done using this pulverized powder and high- Temperature fermentability yeast Schizosaccharomyces japonicus S S4-5 at 4 0 °C, ethanol production was inhibited. To improve the efficiency of ethanol production under high temperature condition, SS4-5 was subjected to UV irradiation. As the result of mutation, we obtained stable ethanol producing mutant, named SS4-5SP, under high temperature condition. When SSF was done using SS4-5SP at 40 °C, ethanol production and ethanol yield were 51.11 g/L and 81.81 %, respectively. High efficiency ethanol production can be achieved by SSF using SS4-5SP.