Development and method validation for determination of Deltamethrin residue in olive oil using a reversed-phase high performance liquid chromatography

Abstract

Olive oil is the most important commodities produced in the Mediterranean region. Due to its significant economical importance, the usage of pesticides in its production is systematic, by using a wide range of plant protection products with a variety of modes of action. As a consequence, monitoring of their residue levels in these products is a necessity. In the present study a reversed-phase high performance liquid chromatography method, with a short sample preparation step, based on acetonitrile extraction is developed and validated in olive oil, with a large scope that includes Deltamethrin as pesticide. Good sensitivity and selectivity of the method were obtained with limits of quantification at 0.2 mg kg-1. Deltamethrin has recovery rate which is of about 80℅. We confirm also the efficiency of alumina, used as adsorbent in the clean up step, to remove triglycerides and to get a pure extract. The agronomic implementation of this protocol allows us to determine the influence of some parameters on the dose and the period of treatment affecting the detected quantities of Deltamethrin residues in the produced olive oil. Indeed, we prove that the treatment dose should be specific for each case considering the olive variety, the geography of the orchard, and the predicted harvest time to determine the convenient dose of treatment. In addition, the results show that the preventive treatment at the blooming phase, does not lead to the concentration of Deltamethrin residues in the oil as it happens at the lipogenesis phase.