Biocatalyst: Phytase production in solid state fermentation by ovat strategy

Abstract

The phytase-producing Pichia membranifaciens S3 (MG663581) was isolated from sugarcane juice using phytase screening medium. The predicted and experimental model showed maximum phytase production of 364 U/ml appearing g/100ml: Jack fruit seed (1.5), peptone (0.15), dextrose (0.50), yeast extract (0.05), malt extract (0.05) pH 5.5 and 28°C) used OVAT strategy by Solid state fermentation through shake flask methodology. The modelling, 3D structure of Phy S3 amino acid sequences was modelled (PhyS3.B99990003) by using Modeler 9.23 and validated results showed that 86.4% in the favoured region by Ramachandran plot.