HF-IDS-Cam: Fast video capture with ImageJ for real-time analysis

Abstract

Fast online video analysis is currently a key issue for dynamic studies in biology; however, very few tools are available for these concerns. Here we present an ImageJ plug-in: HF_IDS_Cam, which allows for video capture at very high speeds using IDS (Imaging Development Systems GmbH) cameras and image analysis software ImageJ. The software has been optimized for real time video analysis with ImageJ native function and other plug-ins and scripts. The plug-in was written in Java and requires ImageJ 1.47v or higher. HF_IDS_Cam offers a wide range of applications for exploration of dynamic phenomena in biology, from in vitro/ex vivo studies, such as fast fluorescent calcium imaging and voltage optical mapping in cardiac myocytes and neurons, to in-vivo behavioral studies.