Andrology laboratory review: evaluation of sperm motility

Abstract

This review is an effort by the andrology taskforce of the Association of Applied AnimalAndrology, American College of Theriogenologists, European College of Animal Reproduction, andSociety for Theriogenology. It is intended to serve as a reference on methods to evaluate spermmotility in domestic animals and to contribute to adoption of best practices in veterinary andrologylaboratories and semen processing centers. Sperm motility evaluation topics covered include samplepreparation, subjective evaluation, computer-assisted semen analysis, and use of sperm qualityanalyzers. Emphasis is given to principles of the methods, equipment, performing evaluation, andcommon mistakes and/or pitfalls. In addition, precision and accuracy of the various methods arediscussed.