Construction of specific erythromycin resistance mutations in the temperate lactococcal bacteriophage TP901-1 and their use in studies of phage biology

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Abstract

A method for the construction and isolation of specifically designed mutations of the temperate lactococcal phage TP901-1 has been developed. Two different erm-labeled mutants were isolated. One was shown to be defective in lysogenization and excision. The other, showing normal lysogenization, was used for host range studies.

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Koch, B., Christiansen, B., Evison, T., Vogensen, F. K., & Hammer, K. (1997). Construction of specific erythromycin resistance mutations in the temperate lactococcal bacteriophage TP901-1 and their use in studies of phage biology. Applied and Environmental Microbiology, 63(6), 2439–2441. https://doi.org/10.1128/aem.63.6.2439-2441.1997

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