Monitoring enzymatic ATP hydrolysis by EPR spectroscopy

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Abstract

An adenosine triphosphate (ATP) analogue modified with two nitroxide radicals is developed and employed to study its enzymatic hydrolysis by electron paramagnetic resonance spectroscopy. For this application, we demonstrate that EPR holds the potential to complement fluorogenic substrate analogues in monitoring enzymatic activity. © Partner Organisations 2014.

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Hacker, S. M., Hintze, C., Marx, A., & Drescher, M. (2014). Monitoring enzymatic ATP hydrolysis by EPR spectroscopy. Chemical Communications, 50(55), 7262–7264. https://doi.org/10.1039/c4cc02422b

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