Flavin mononucleotide-based fluorescent protein as an oxygen-independent reporter in Candida albicans and saccharomyces cerevisiae

Abstract

Hypoxia is encountered frequently by pathogenic and apathogenic fungi. A codon-adapted gene encoding flavin mononucleotide-based fluorescent protein (CaFbFP) was expressed in Candida albicans and Saccharomyces cerevisiae. Both species produced CaFbFP and fluoresced even during hypoxia, suggesting that oxygenindependent CaFbFP is a useful, novel tool for monitoring hypoxic gene expression in fungi. Copyright © 2009, American Society for Microbiology. All Rights Reserved.