Biotechnology methods to produce planting material of the genus Rubus L.

Abstract

The work is devoted to improving clonal micropropagation methods of the genus Rubus representatives. When cultivating R. arcticus L. cultivars, the optimal concentration of 6-benzylaminopurine (6-BAP) was 0.3 mg L-1. Significant effect of FeEDTA concentration in the nutrient medium on multiplication of R. idaeus L. cultivars was established. The interaction of auxin type and plant genotype was revealed during rooting of blackberry cultivars and raspberry-blackberry hybrids. The nutrient medium supplemented with 0.5 mg L-1 indoleacetic acid contributed to the highest percentage of rhizogenesis in R. arcticus representatives (82%). The optimal growth regulator for conservation of raspberry explants was 6-BAP at a concentration of 0.3 mg L-1. One of the most representative in vitro collections of Rubus cultivars has been created.