Hypoxic Preconditioning Effect on the Expression of Intracellular Heat Shock Protein (HSP) 27, HSP 70 and HSP 90 on Cultured Adipocyte-Derived Mesenchymal Stem Cells (AMSCs)

Abstract

New hope in the treatment of myocardial infarction has emerged by transplanting multipotent stem cells into the affected myocardium. Hypoxic preconditioning (HPC) is expected to increase the survival cell potency by inducing upregulation of Heat Shock Protein (HSP) in Adipocyte-derived Mesenchymal Stem Cells (AMSCs). This study aimed to investigate the roles of HPC in AMSCs to observe the in vitro expression of HSP27 as an ATP independent chaperone, and HSP70, HSP90 as an ATP dependent chaperone. This study is a true experimental randomized post-test design study. AMSCs were isolated from adipose tissue and cultured until there were four passages. The samples were then divided into two groups, HPC (O2 1%) and normoxia (O2 21%). Immunocytofluorescence techniques were used to evaluate the expression of HSP27, HSP70, and HSP90. Data were analyzed using a two paired t-test and Mann Whitney U test. There was a significant increase of HSP27, HSP70, and HSP90 expression in HPC compared to the normoxia group (272,00±92,225 vs 86,19±26,362 p<0.0001; 130,88±45,416 vs 120,75±97,113 p<0.05; 165,75±58,930 vs 96,81±22,578). Hypoxic preconditioning significantly increased the expression of HSP27, HSP70, and HSP90 on cultured AMSCs.