Background: Anti-CD3 therapy can induce immunosuppression by several non mutually exclusive mechanisms that have been proposed to explain the therapeutic effect the administration anti-CD3 mAb, but its immunoregulatory mechanism is still not completely clear. In T cells, microRNAs (miRNAs) regulate several pathways, including those associated with immune tolerance. Here, we report changes in miRNA expression in T cells following treatment with anti-human CD3 antibodies. Peripheral blood mononuclear cells were cultured in the presence of the monoclonal antibody OKT3 or a recombinant fragment of humanized anti-CD3. Following these treatments, the expression profiles of 31 miRNA species were assessed in T cells using TaqMan arrays. Results: Eight of the tested miRNAs (miR-155, miR-21, miR-146a, miR-210, miR-17, miR-590-5p, miR-106b and miR-301a) were statistically significantly up- or down-regulated relative to untreated cells. Conclusions: Stimulation of T cells with anti-human CD3 antibodies alters miRNA expression patterns, including of miRNA species associated with immune regulatory pathways.
CITATION STYLE
Sousa, I. G., Do Almo, M. M., Simi, K. C. R., Bezerra, M. A. G., Andrade, R. V., Maranhão, A. Q., & Brigido, M. M. (2017). MicroRNA expression profiles in human CD3+ T cells following stimulation with anti-human CD3 antibodies. BMC Research Notes, 10(1). https://doi.org/10.1186/s13104-017-2442-y
Mendeley helps you to discover research relevant for your work.