Magnesium isoglycyrrhizinate protects against renal-ischemia-reperfusion injury in a rat model via anti-inflammation, anti-oxidation and anti-apoptosis

Abstract

The present study aimed to investigate whether magnesium isoglycyrrhizinate protects against renal ischemia-reperfusion injury (RIRI), and to verify the underlying mechanisms. An RIRI rat model was induced by removing the right kidney, and exposing and clamping the left kidney. RIRI model rats were administered 30 mg/kg magnesium isoglycyrrhizinate for 3 days. Blood urea nitrogen (BUN) and serum creatinine levels in the blood of RIRI model rat were examined, compared with sham-operated controls. Magnesium isoglycyrrhizinate suppressed the activities of tumor necrosis factor-α, interleukin (IL)-1β, IL-6, superoxide dismutase, glutathione peroxidase, inducible nitric oxide synthase (iNOS) and caspase-3 in RIRI model rats. Renal iNOS, matrix metalloproteinase (MMP)-2, phosphorylated-signal transducers and activators of transcription 3 (STAT3) and intercellular adhesion molecule-1 (ICAM-1) protein expression levels were suppressed by magnesium isoglycyrrhizinate treatment in RIRI model rats. These findings suggested that magnesium isoglycyrrhizinate protects RIRI via anti-inflammatory, -oxidative and -apoptotic mechanisms in an RIRI rat model. These results implicate magnesium isoglycyrrhizinate pretreatment as a potential approach to protect against RIRI via suppression of the iNOS, ICAM-1, MMP-2 and STAT3 signaling pathways.