Time-related changes in periodontal mechanoreceptors in rat molars after the loss of occlusal stimuli

Abstract

The effect of a loss of occlusal stimuli upon the distribution and structure of the periodontal mechanoreceptors of the rat mandibular molar was examined after extracting opposing molars. The hypofunctional periodontal ligament narrowed significantly two weeks after tooth extraction, associated with an altered morphology of the Ruffini endings that showed typical dendritic profiles in normal controls. At four weeks and later periods after extraction, the Ruffini endings - including those without light microscopic changes - demonstrated unusual ultrastructural features such as the eccentric localization of mitochondria along the axonal membrane and loss of other cell organelles, unusual elongation of axonal microprojections, or a deep invagination of the Schwann sheath into the axoplasm. Immunoreactivity for the growth-associated protein-43 (GAP-43) in the Ruffini endings was restricted to the Schwann element in both the normal and hypofunctional periodontal ligament, but the reaction was weaker and even negligible in some cases in the latter ligament. The present results suggest that occlusal stimuli are essential for maintaining the structural integrity of the periodontal ligament, including that of periodontal mechanoreceptors. A decreased immunoreactivity for GAP-43 in the Schwann sheaths supports the notion of a possible functional alteration in the Ruffini endings that showed no structural abnormality.