The optimization of ultrasonic method for the extraction of Pumpkin seed (Cucurbita maxima)

Abstract

The purpose of the present study was to determine the optimal ultrasound-assisted extraction (UAE) conditions (sample-to-solvent ratio, ultrasonic power, and time) for the extraction of pumpkin seed oil.  Pumpkin seed oil was analyzed for yield, 2.2’-Diphenyl-1-picrylhydrazyl (DPPH) scavenging activity, and total phenolic content.  Soxhlet extraction methods were assessed for comparison.  The Box Behnken design (BBD) was applied to optimize UAE conditions.  The optimum UAE conditions were obtained using sample-to-solvent ratio of 1:10 mL g-1, ultrasonic power of 150 Watt in 15 min extraction time.  Compared with Soxhlet extraction methods, UAE exhibited lower oil yield and total phenolic content but had higher DPPH scavenging activity.  The advantage of ultrasonic-assisted extraction was clearly reduction of extraction duration.