Pyrrolysine analogs as substrates for bacterial pyrrolysyl-tRNA synthetase in vitro and in vivo

Abstract

Pyrrolysine-tRNA Pyl complex is produced by pyrrolysyl- tRNA synthetase (PylRS). In this study, we investigated the substrate specificity of Desulfitobacterium hafnience PylRS. PylRS incorporated various L-lysine derivatives into tRNA Pyl in vitro. In addition, the PylRS/ tRNA Pyl pair introduced these lysine derivatives into the recombinant protein by the Escherichia coli expression system, indicating that this PylRS/tRNA Pyl pair can be used in protein engineering technology.