Role of Plant Growth Regulators (Auxin and Cytokinin) in Callus Induction in Rice (Oryza sativa L.) C.V. DM-25

Abstract

Methods were developed to optimize the media for callus induction from mature embryos of indica rice ( Oryza sativa L.) CV. DM-25. Callusing from seeds of rice was observed with various levels of 2,4-d alone and in combination with different concentrations of benzyl adenine. With the gradual increase of 2,4-d concentration in callus induction medium, there was gradual increase in callusing frequency.