Multi-Color Fluorescence in situ Hybridization

Abstract

Fluorescence in situ hybridization (FISH) is an experimental technique used in chromosome analysis. In situ hybridization (ISH) for chromosomes has been used for over 40 years. The development of a non-radioactive fluorescent detection system enabled the identification of multiple specific nucleotide sequences on chromosomes. Multi-probe detection further increased the speed of chromosome analysis. Here, we describe the use of FISH in plant chromosomes, from the labelling of the probes to multi-color detection and the creation of multi-color FISH images.