Etude de la sensibilité aux antibiotiques des mycoplasmes isolés de spermes à Abidjan

Abstract

Mycoplasma urogenital tract bacteria that are often associated with male infertility. At the therapeutic level, acquired resistance to antibiotics has been reported. The objective of this work was to determine the sensitivity level of urogenital mycoplasma strains isolated during sperm culture. Retrospective study focused on 313 mycoplasma identified with Mycoplasma IST2 kit (Biomérieux Ref 42 505). This kit allows differential quantification, identification of Ureaplasma urealyticum, and Mycoplasma hominis, as well as sensitivity studies to nine antibiotics (josamycin, azithromycin, erythromycin, clarithromycin, pristinamycin, tetracycline, doxycycline, ofloxacin and ciprofloxacin). Ureaplasma urealyticum was identified in 245 samples (78.3%) and Mycoplasma hominis in 27 (8.6%). Co-infection (Ureaplasma urealyticum/Mycoplasma hominis) was found in 41 patients (13.1%). Josamycin was active on all germs with sensitivities of 90.2% and 92.6% respectively for Ureaplasma urealyticum and Mycoplasma hominis. Cycline activity on Ureaplasma urealyticum was good while fluoroquinolone activity was poor. Pristinamycin was effective on Mycoplasma hominis (70%). The study showed that josamycin was active on all mycoplasma, cyclins retained good efficacy on Ureaplasma urealyticum unlike fluoroquinolones. These variations in sensitivity justify the interest of identifying the two species and studying the sensitivity for a good management of these infections.