Enhancement of reticuloendotheliosis virus-induced bursal lymphomas by serotype 2 Marek's disease virus

Abstract

The effect of serotype 2 and 3 Marek's disease virus (MDV) vaccines on the pathogenesis of reticuloendotheliosis virus (REV)-induced bursal and non-bursal lymphomas was examined in chickens of RPRL lines 15I5 × 71 and 63 × 0, respectively. At hatch, chickens were vaccinated with strain 301B/1 of serotype 2 MDV or strain FC126 of turkey herpesvirus (HVT), a serotype 3 MDV and inoculated with spleen necrosis virus (SNV), a non-defective strain of REV. In another experiment, bursas from 14-week-old 15I5 × 71 chickens coinfected with strain 301B of serotype 2 MDV and SNV strain of REV at hatch were examined microscopically for REV-induced transformed follicles by methyl green pyronin stain and for the presence of MDV by in situ hybridization. The incidence of REV-induced bursal lymphomas was significantly higher in 15I5 × 71 chickens vaccinated with serotype 2 MDV than in unvaccinated chickens or chickens vaccinated with HVT. On the other hand, the incidence of REV induced nonbursal lymphoma in chickens of line 63 × 0 vaccinated with serotype 2 MDV was comparable to that in unvaccinated chickens or chickens vaccinated with HVT. The average number of hyperplastic follicles in bursas from REV-infected 15I5 × 71 chickens was significantly higher in chickens vaccinated with serotype 2 MDV than that in unvaccinated chickens or chickens vaccinated with HVT, and the MDV was more frequently detected in REV-transformed than in untransformed bursal follicles. Data from this study suggest that serotype 2, but not serotype 3, of MDV may enhance the development of REV-induced bursal lymphomas, and that neither serotype 2 nor serotype 3 MDV influence the development of REV-induced nonbursal lymphomas. The data also suggest that the enhancement effects of serotype 2 MDV on REV bursal lymphomas may be at the stage of formation of hyperplastic or transformed bursal follicles.