Cell counting chamber vs. Sysmex XN-1000 for determining white blood cell count and differentiation for body fluids

Abstract

Objectives: Sterile body fluids (BFs) include key information for the diagnosis and monitoring of a variety of diseases. A cornerstone test is the total white blood cell (WBC) count, which comprises the differential of WBC of body fluid analysis. It is important to test automated hematology analyzers that should be verified using patient samples. The aim of this study is to compare both the performance of the Sysmex XN-1000 system's body fluid module for cell counting and differentiation to the results of a cell counting chamber. Methods: This study was performed on 200 routinely laboratory sent BFs. Cell counts and differentiation were determined with both bright-lined Neubauer Cell Counting Chamber and Sysmex XN-1000 system body fluid mode. Results: The correlation coefficients of WBC count by two methods indicated very strong correlation (r≥0.90, p<0.0001) for any specimen except pleural fluid. According to Passing-Bablok regression analysis, Sysmex XN-1000 showed acceptable performance according to bias of the slope criteria (