In-vitro Free Radicals Scavenging and Antioxidant Activity of Rice Bran Extract

Abstract

ABSTRACT Aim:To evaluate the antioxidant activities of the γ-oryzanol isolated from rice bran oil of Indian origin in various in vitro models. Materials and Methods: The hydrogen donating ability of the hydroalcoholic extract of γ-oryzanol was measured in the presence of 1, 1-diphenyl-2-picryl-hydrazyl (DPPH) radical. Results: A 100 μg mLG 1 of γ-oryzanol and ascorbic acid exhibited 64.56 and 64.88% inhibition, respectively and the IC 50 values were found to be 48 and 56.50 μg mLG 1 for γ-oryzanol and ascorbic acid respectively. The effect of γ-oryzanol on reducing power was studied according to the reaction of Fe ). Moreover, the results were observed in a concentration dependent manner. Conclusion: All the above in-vitro studies clearly designate that the hydroalcoholic isolated γ-oryzanol has a significant antioxidant activity.