Chemical profile, toxicity, cytotoxicity and antioxidant potencies of ethylacetate extract of soft coral Lobophytum sp. growing in South East Sulawesi Sea

Abstract

Soft coral of Lobophytum sp. grows a lot in the seas of South East Sulawesi, Indonesia. However, no information has been found regarding the study of chemical and pharmaceutical aspects of this genus from this area. Therefore, the purpose of this article is to present the results of a general study on the pharmaceutical and chemical aspects of Lobophytum sp. The sample was collected from the water of Saponda Island, and than extracted by ethyl acetate. The chemical content was analyzed using phytochemical tests and LCMSMS. Antioxidant potency was evaluated by DPPH radicals and ABTS methods, cytotoxicity towards MCF7 cell line using MTT assays, and toxicity by BSLT. The results showed that the ethylacetate extract has potential as an antioxidant and cytotoxic against MCF7 cell lines which is indicated by IC50 value. The antioxidant potential was revealed by IC50 (g/L) 84.99 (DPPH), and 67.99 (ABTS). In addition, the cytotoxicity presented by IC50 (g/L) 37.35 and 50.07 for BSLT. Those activities were supported by the qualitative phytochemical screening that exhibited the extract contains terpenoids, steroids, alkaloids and phenolics compounds. LCMSMS data indicated that ethylacetate extract has arachidonic acid, edultin, fragransol D, neociwujiaphenol, pluviatelol, and some unidentified compounds with molecular formulas C21H26O8, C23H47N3O, C32H51NO7, C31H47NO3, C33H51NO3.