Performance of Three Pneumatic Probe Samplers and Four Analytical Methods Used to Estimate Aflatoxins in Bulk Cottonseed

Abstract

The requirement by the U.S. Food and Drug Administration that agricultural products susceptible to aflatoxin contamination contain aflatoxin at levels ≤20 parts per billion for consumer-ready products has led to the establishment of inspection programs by various industries. In Arizona, cottonseed samples from 100 ton piles are collected by an accumulation of 3 or more probings with a pneumatic probe. When sampling compacted cottonseed piles, the large official pneumatic probe (7.6 × 127 cm) decreases in efficiency. Two smaller probes (1.9 × 127 cm and 1.9 × 254 cm) were therefore developed and tested for their suitability for sampling cottonseed piles. Three rapid analytical methods (one thin-layer Chromatographie and 2 immunochemical) were tested for suitability as on-site assay systems. An analysis of variance of the analytical test results showed no differences between the various probes tested. Of the rapid methods, however, only the AflaTest-P immunoaffinity column gave resimilar to those of the official AOAC thin-layer chromatography method. In terms of safety, however, all methods prevent material contaminated above regulatory limits from reaching the consumer.