Isolation of Secondary Metabolites from Ipomea Digitata L. Tuber Extracts and it’s Effect on Cardiovascular Risk Parameters

Abstract

The objective of present investigation was to test the presence of various phytochemicals present in the ethanol extract (EE) of Ipomea digitata L. tuber and to isolate the active constituents present in the EE. Further it was aimed to evaluate the antioxidant activity using in vitro model and to assess the effects of extracts of Ipomea digitata L. on risk factors associated with cardiovascular disorders. Phytochemical tests were performed. After performing column chromatography, the isolated compounds were characterized by IR, H-1 NMR, C-13 NMR and MS techniques. For antioxidant activity, 1,1-dipheny1-2-picrylhydrazyl (DPPH) method, Nitric oxide (NO) method and hydrogen peroxide (H2O2) methods were used. The extraction yield for EE was found to be 6.66% and the extract was light brown in appearance with semisolid consistency. Two phytoconstituents of category viz. phytosterol (beta-sitosterol) and a coumarin (7-Hydroxy-4-methylcoumarin) were isolated and characterized. The findings suggested that the antioxidant activity of EE is in dose dependent manner. Cholesterol rich diet fed to animals caused increased Albumin, Cholesterol and LDL-Cholesterol. Cholesterol rich diet also reduced the concentration of Serum Globulin, HDL-Cholesterol and Triglycerides (TG) when comparison was made with control group. Dosing of EE (200 mg/kg b.w.) caused increased level of serum TG, HDL-Cholesterol and reduced level of LDL-Cholesterol when comparison was made with control, in the cholesterol rich diet offered rats. The result concluded the presence of number of secondary metabolites in EE of Ipomea digitata L. Moreover, the present paper reports the presence of beta-sitosterol and 7-Hydroxy-4-methylcoumarin in the EE of Ipomea digitata L. very first time. Finding also suggests that EE may be explored as functional food in near future.