Ultrasound-Triggered Mg2+ Blasting Release Hydrogel Microspheres for Promoting Bone Reconstruction

Abstract

Mg2+ (Magnesium ion) can affect bone tissue metabolism by regulating related signaling pathways in bone metabolism. However, how to realize precise controlled release of Mg2+ in bone regeneration treatment still presents a challenge. Herein, for the first time, the GelMA-BP (Gelatin Methacryloyl-Bisphosphonate) and the composite nano-bubble system are fused to construct the Mg2+ blasting controlled-release hydrogel microspheres, the stability of the nano-bubbles in the microspheres is enhanced through metal coordination complexation, and the burst of the nano-bubbles is controlled by using ultrasonic cavitation to achieve the precise controlled release of Mg2+, ultimately effectively promoting bone reconstruction. First, GelMA-BP composite is prepared by Schiff base reaction. Second, the nanobubble BP (Bisphosphonate) system is constructed, and Mg2+ is combined with the ligand coordination to prepare the composite nanobubble system. Thirdly, through Mg2+ co-coordination, the ultrasound-triggered Mg2+ blasting controlled release microspheres were prepared to achieve bone repair. Overall, this innovative strategy effectively solves the problem of accurate controlled release of Mg2+, and finally effectively activates in situ bone tissue regeneration.