Abstract
Bovine serum albumin (BSA) was used as a stabilizing agent and biofunctionalized layer for water-dispersed gold nanoparticles (NPs) synthesized from metal precursor HAuCl4. The BSA binding to gold NPs was characterized qualitatively and quantitatively by transmission electron microscopy, UV-VIS and FTIR spectrophotometers. HER2 (human epidermal growth factor receptor 2) specific phage antibodies were attached to BSA stabilized gold NPs to form a gold-antibody complex. An ELISA (enzyme-linked immunosorbent assay) test was done to confirm the bioactivity of antibodies attached to gold NPs. © 2010 Vietnam Academy of Science & Technology.
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Nghiem, T. H. L., La, T. H., Vu, X. H., Chu, V. H., Nguyen, T. H., Le, Q. H., … Tran, H. N. (2010). Synthesis, capping and binding of colloidal gold nanoparticles to proteins. Advances in Natural Sciences: Nanoscience and Nanotechnology, 1(2). https://doi.org/10.1088/2043-6254/1/2/025009
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