Synchronized variations in levels of essential amino acids during germination in grain Amaranth

Abstract

Seed germination is a fundamental process involving series of biochemical changes contributing to successful growth and development of new plant. Changes in the content observed during germination reflect developmental stages. Analyses of their behavior and profiles could provide insights into different strategies for regulating metabolism. In present study, seeds of A. hypochondriacus were germinated at controlled conditions and were used for estimation of total proteins and free amino acids. The estimation of amino acids was done at different time intervals throughout germination using LC MS/MS technique. The trends observed in content of individual free amino acids showed essential amino acid share a similar pattern of metabolism indicating coordinated regulation of amino acid pathways.