Antioxidant and Anti-Inflammatory activity of Chlorogenic Acid on Lead-Induced Fibroblast Cells

Abstract

Lead (Pb) can be a cause of oxidative stress, because it is a toxin found in the environment and can have a harmful effect on the body. increased reactive oxygen species (ROS) and nitrogen species (RONS) levels can cause cellular aging and inflammation. natural compound found in plant is one of the sources of antioxidant and anti-inflammatory agent that has ability to prevent aging including chlorogenic acid (CA). Cytotoxicity assay of CA against fibroblast cells (BJ) was conducted using MTS (3-4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium). The intracellular ROS levels were detected by flow cytometry using a DCF-DA fluorescent probe. BJ cells were incubated at 37°C, 5% CO2, treated by 25 and 6.25 μg/ml CA for 4 h and followed by 400 μg Pb for 3 days. The anti-inflammatory potential was determined using ELISA to measure IL10 and TNFα. CA at 3.13-25 μg/ml were nontoxic to the BJ cells. CA treatment at 6.25 and 25 μg/ml was capable to reduce the accumulation of ROS in lead-induced BJ cells. CA at 6.25 and 25 μg/ml increased IL10 and reduced TNFα compared to positive control (lead-induced cells). This study shows that chlorogenic acid has the potential as protective effect through suppression of ROS levels and have anti-inflammatory properties related to Pb poisoning.