Characterization of digestive enzymes from sandfish, Holothuria scabra juvenile

Abstract

Understanding the specific role of enzyme activity, such as digestive enzymes present in the intestine of Holothuria scabra juvenile, combined with an understanding of the animal habits and their digestive capacity is essential in the development of an appropriate diet. Juvenile sandfish with an average weight and total length of 2.81 ± 0.54 g and 4.10 ± 0.54 cm were collected and its intestine was used to analyze the activity of protease, amylase, and lipase enzymes. Parameters testing for all enzyme activities were conducted at 12 levels of pH (1.0; 2.0; 3.0; 4.0; 5.0; 6.0; 7.0; 8.0; 9.0; 10.0; 11.0; 12.0) and temperature 8 levels (10; 20; 30; 40; 50; 60; 70; 80; 90°C). Optimum pH for protease, amylase, and lipase activity were 7.0, 8.0, and 3.0, respectively, while optimum temperatures were 50, 50, and 40°C, respectively. This information about optimum pH and temperature in the biology of sandfish is useful as a basis for further studies on feeding physiology.