Purification and characterization of microbial gellan lyase

Abstract

Gellan lyase was purified from the culture fluid of soil samples incubated in a medium containing gellan as a sole carbon source. The enzyme was a monomer with a molecular mass of 140 kDa and was must active at pH 7.5 and 45°C. The enzyme was highly specific to gellan and lowered the viscosity of the polymer.