Prevalence and Multiplex PCR for Enterotoxin Genes of Staphylococcus aureus Isolates from Subclinical Mastitis and Kareish Cheese

Abstract

Aim: Staphylococcus aureus was categorized as a contagious pathogen incriminated in subclinical mastitis as well as in dairy products. From public health view, this organism causing food poisoning outbreaks via contamination of food products with its toxins. These studies highlight the prevalence of staphylococcus aureus among subclinical mastitic cases as well as cheese samples and the dominant enterotoxigenic genes. Methodology: Examination of 100 samples (50 from subclinical mastitis, and 50 kareish cheese) from Sadat city, Menoufia province. Results and interpretation: Bacteriological culturing on selective and specific medium revealed that 26.7% and 30% in subclinical mastitis and kareish cheese respectively were due to S. aureus. Furthermore, multiplex PCR proved to be efficient technique for detection of different enterotoxins genes. The sea, seb and see genes were the most prevalent genes among the tested S. aureus isolates. Although, no detection of sec and sed genes were observed. Conclusion: It can be concluded that molecular characterization of S. aureus and its enterotoxins genes will be beneficial in designing control and preventive measures of S. aureus infection in human and animals.