Isolation and Identification of food borne pathogens from Spoiled food samples

Abstract

Studies were performed to identify various pathogenic bacteria from different sources like; spoiled fruits, vegetables, Dairy products, Bakery products, Poultry products and Spoiled rice were selected from local market of Bagalkot district, Karanataka, India. Collected samples were examined in specific media like Mannitol Salt agar, MacConkey agar and Cetrimide agar. Samples were confirmed by both phenotypic and genotypic characteristics. The pathogenic bacterial isolates such as Pseudomonas spp [23.66%], Staphylococcus aureus [22.76%], Salmonella spp [21.87%], E.coli [22.32%] and Klebsiella spp [8.92%] were present in large amount. The percentage of multidrug-resistant (MDR) isolates was high in numbers and the most prevalent resistant pattern included four different classes of antibiotics; Cephalosporins, Fluoroquinolones, Beta-Lactams and Aminoglycosides.