Structural analysis of the oligosaccharide units of xyloglucan and their effects on growth of COLO 201 human tumor cells

Abstract

The oligosaccharide units of xyloglucans from some fruits were analyzed by enzymatic digestion followed by high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD). The oligosaccharide units of the polysaccharides were XXXG, XXLG, XLXG, XXFG, XLLG, and XLFG [where each (1→4)-β-linked D-glucosyl residue in the backbone is given a one-letter code according to its substituents: G, β-D-Glc; X, α-D-Xyl-(1→6)-β-D-Glc; L, β-D-Gal-(1→2)-α-D-Xyl-(1→6)-β-D-Glc; F, α-L-Fuc-(1→2)-β-D-Gal-(1→2)-α-D-Xyl-(1→6)-β-D-Glc] in an approximate molar ratio of 10:51:4:13:11:11 for kiwi, of 23:3:40:27:2:5 for peach, of 31:1:9:19:1:39 for avocado, of 35:3:12:19:5:26 for apple, of 29:5:4:33:4:25 for fig, of 29:17:1:29:13:11 for pineapple, and of 27:4:8:21:4:36 for mandarin. In addition, different types of xyloglucan oligo- and polysaccharides were tested for effects on the growth of COLO 201 human tumor cells. The cell growth was reduced by fucose-containing oligo- and polysaccharides. © 2001, Elsevier B.V. All rights reserved.