Phytochemical Analysis and Antibacterial Activity of Annona muricata (Laxman phal) against ESBLs Producers (Escherichia coli and Klebsiella pneumoniae)

Abstract

The use of plant products as medicine has emerged from traditional days to modern times of medicine in order to improve the quality life of the patients worldwide. Nature-derived medicines are considered safer with less or no side effects. The antibacterial effect of the leaves of Annona muricata was evaluated on bacterial strains of ESBLs: Escherichia coli and Klebsiella pneumoniae are tested which were isolated from D.Y. Patil Hospital, Nerul, NaviMumbai. Std. strain of Escherichia coli ATCC 25922 has been tested for cephalosporin (Ceftazidime- zone of inhibition: 31mm) and Imipenem (zone of inhibition: 25 mm) and is found to be sensitive. The solvents used for the extn. of plants were water (aq.) and ethanol. The antibacterial activity was done by using Kirby-Bauer disk diffusion method. Both exts. showed antibacterial properties but the ethanolic ext. was more effective as it inhibited a wide range of organisms at varying concns. The most susceptible Gram-neg. bacteria was Escherichia coli. The significant antibacterial activity of plant exts. was compared with the std. antibiotic, Imipenem. The results obtained in the present study suggest that Annona muricata can be used in treating diseases caused by the test organisms and or can be used along with the antibiotics having synergistic activity. [on SciFinder(R)]