Chymotryptic digestion of Tetrahymena ciliary dynein. II. Pathway of the degradation of 22S dynein heavy chains

Abstract

As shown in the preceding paper (Toyoshima, Y.Y., 1987, J.Cell Biol., 105:887-895) three-headed Tetrahymena 22S dynein consists of three heavy chains (HCs) and is decomposed into two-headed (H) and one-headed (L) fragments by chymotryptic digestion. To accurately determine the presence of multiple ATPases and ultimately the location of various domains, it is necessary to determine the identity of each HC fragment relative to the original HCs in 22S dynein. The degradation pathway of each HC was determined by peptide mapping and immunoblotting. The three HCs (Aα, Aβ, and Aγ) were immunologically different; although SDS-urea gel electrophoresis showed that Aγ HC was apparently resistant to the digestion, actually three distinct HCs contributed to the same band alternately. H fragment was derived from Aβ and Aγ, whereas L fragment originated from Aα HC. Since both fragments were associated with ATPase activity, these results directly demonstrate the presence of multiple ATPase sites in Tetrahymena 22S dynein.