Potentiometric determination of cholesterol-reducing drug, ezetimibe using coated wire membrane sensors

Abstract

Newly developed coated wire membrane electrodes have been developed for determination of ezetimibe (EZ) in pure form, pharmaceutical preparations and in biological fluids. The selective electrodes were based on the incorporation of ezetimibe with the ion exchanger(s) phosphotungstic acid (EZ-PTS), phosphomolybdic acid (EZ-PMO) and a mixture of both (EZ-PTS/PMO). The electrodes exhibit linear response with a Nernstian slope (57.37 ± 0.235, 55. 40 ± 0.748 and 58.04 ± 0. 536 mV decade -1 at 25 °C) over concentration range 1.0 ×10 -6 - 1.0 × 10 -1, 1.0×10-6-1.0 × 10 -2 and 1.0 × 10 -6 - 1.0 × 10 -1 mol L -1 for the three electrodes, respectively. The working pH range of the proposed electrodes was 3-7. The influence of possible interfering species such as common inorganic cations, many sugars, amino acids and different pharmacological related compounds was studied. Statistical student's t-test and F test showed insignificant systematic error between proposed and reported methods. Copyright © 2011 American Scientific Publishers All rights reserved.