Comparative analysis of conventional PCR and real-time PCR to diagnose shrimp WSD

Abstract

The aims of this study were to standard and optimize a qPCR protocol with FAM-BHQ1 probe, and to compare its sensitivity against TaqMan qPCR and PCR methods to diagnose shrimp WSD. The FAM-BHQ1 qPCR presented higher clinical sensitivity and showed to be a robust alternative to detect WSSV in clinical samples. © 2013, Sociedade Brasileira de Microbiologia.