Evaluation of a quantitative plasma PCR plate assay for detecting cytomegalovirus infection in marrow transplant recipients

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Abstract

A plasma PCR test, using a nonradioactive PCR plate assay, was evaluated for detection of human cytomegalovirus reactivation. This assay was compared to Southern blotting and found to perform well. As a noncompetitive method of quantitation, it was similar to a competitive method for detecting the number of genome copies per milliliter of plasma in marrow transplant recipients. This is a technically simplified assay with potential for adaptation to automation.

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Gallez-Hawkins, G. M., Tegtmeier, B. R., Ter Veer, A., Niland, J. C., Forman, S. J., & Zaia, J. A. (1997). Evaluation of a quantitative plasma PCR plate assay for detecting cytomegalovirus infection in marrow transplant recipients. Journal of Clinical Microbiology, 35(3), 788–790. https://doi.org/10.1128/jcm.35.3.788-790.1997

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