Evaluation of crude ethanolic extract of aquatic plants as inhibition zone against Flavobacterium columnare

Abstract

Three different aquatic plants Selaginellaplana (Desv. Ex. Poir) Hieron, Ludwigiaadscendens (L.) H. Hara, dan Ephitemabenthamii C. B. Clarke were collected from South Sulawesi and crude extracts all of plants parts were prepared in ethanol. This extracts were checked for the presence of inhibitory zone by agar well diffusion method against gram negative Flavobacteriumcolumnare bacterial. Furthermore Minimum Inhibitory Concentration (MIC), Minimum bactericidal concentrations (MBC) testing has been done for crude extract of plants. Extracts of three plants prepared in ethanol showed relatively inhibitory zone to Flavobacterium columnareand two extracts of above plants were positive in MIC and MBC.