Chitin from the extract of cuttlebone induces acute inflammation and enhances MMP1 expression

Abstract

We previously reported that the extract from cuttlebone (CB) has wound healing effect in burned lesion of rat. In present study, the main component of CB extract was analyzed and its wound healing activity was evaluated by using in vitro acute infl ammation model. The extract of CB stimulated macrophages to increase the production of TNF-α. The extract also enhanced the production of TGF-β and VEGF, which were involved in angiogenesis and fi broblast activation. The treatment with CB extract enhanced proliferation of murine fi broblast. CB extract also induced the activation of fi broblast to increase the secretion of matrix metalloproteases 1 (MMP1). The constituent of CB extract which has wound healing activity was identifi ed as chitin by HPLC analysis. The mechanism that the CB extract helps to promote healing of burned lesion is associated with that chitin in CB extracts stimulated wound skins to induce acute infl ammation and to promoted cell proliferation and MMP expression in fi broblast. Our results suggest that CB or chitin can be a new candidate material for the treatment of skin wound such as ulcer and burn. © 2013 The Korean Society of Applied Pharmacology.